Flow cytometry is a procedure that is used to determine and measure the chemical and physical qualities of particles or a population of cells. The individual doing the sampling will put the fluid that has the particles in a liquid that is added in a flow cytometer and then pass it through a laser. Such services are popular in clinical trials and basic research. The following are some of the tips to help you get the best flow cytometry services.
You must be aware of the services you need. Flow cytometry has multiple areas that a person can specialize in. You need to be familiar with your field of interest so that you get a professional specializing in the same field. Among the services that a person can get are things like counting cells, sorting cells, protein engineering detection, and diagnosing disorders like blood cancer.
Find multiple laboratories so that you can make a comparison. You have a higher likelihood of getting the best service provider if you make a comparison of the available options. Request your friends and relatives to give you recommendations of some of the professionals they have worked within the past and lookup for other options online. Narrow down to a few options from the many choices you have found and then start evaluating them. Make sure that you have sufficient measures that you can use to find the best option.
Identify the best alternative from your choices. Getting an exemplary laboratory from your alternatives will depend on the aspects to consider during evaluation. You must take into account things like the type of instrument that the company has, their level of experience as well as accreditation. You can determine whether the service provider is experienced by looking at the reputation they have in the industry and how long we have been offering these services.
Make sufficient plans for the experiment. You must make the required preparation if you want the process to be successful. Verify that the reagents required for the process are available and you have adequate fluorophores to perform the laser process. In addition to that, you must have a hypothesis of the results at the back of your mind. Also, you might want to consider the likelihood of cellular changes and components that might be an obstacle to the elements.
Get yourselves ready. Your cell preparation will be based on the research you have conducted. Nevertheless, there are several preparation tips for many sample types. They include things like cell culturing, utilizing anticoagulants to prepare the blood for evaluation among others. If you are losing bone marrow cells, you might want to get rid of all muscles and tissues because they can block the cytometer flow.
Find different measures to control an experiment. You must put in place necessary control precautions to come up with meaningful data in the long run. You can easily establish the positives and the negatives from the process. Examples of control methods that you can use as they are sustained method, single stained method, and fully stimulated. The fully stimulated is the best for finding out about the population of the cells.